The present study illustrates that ER stress induced by spinal cord injury plays a detrimental role on blood-spinal cord barrier integrity, on the contrary, autophagy induced by spinal cord injury plays a furthersome role in blood-spinal cord barrier integrity in acute spinal cord injury. Proteins significantly altered (ANOVA and Tukey, p < 0.01) are expressed as a ratio of spot volume variations in old adult and senescent rats versus young (6 months) control, respectively. In contrast to light chain 3B (LC3B), the protein expression levels of BECLIN1 and sequestosome 1 (SQSTM1) were increased in the OA group. They were acclimatized under controlled temperature with a 12-h light/12-h dark cycle for a period of one week. Cardiac function and infarct size were measured during/after 125 min of Sunitinib treatment. Upper panel: Histograms of protein expression normalized to the total protein stain (mean±SD). Importantly, the decrease of 8-iso-PGF2α levels correlated with the increased ALDH2 activity at both 24 hours (r = 0.6234, P < 0.001) and 1 week after treatment (r = -0.3941, P = 0.0014). Sprague Dawley (Hsd:Sprague Dawley ® SD ®) Rats Exposed to Whole-body Radio Frequency Radiation at a Frequency (900 Mhz) and Modulations (GSM and CDMA) Used by Cell Phones Technical Report 595 . SAS Sprague Dawley Rat Details. in male and female F344 and Crl:CD (Sprague-Dawley [SD]) rats are rare. Hence, this study aimed to investigate the anti-obesity activity of Elateriospermum tapos (E. tapos) shell extract in obesity induced Sprague Dawley rats. – Chiu, T. Spontaneous Hypertrophic Foci of Pancreatic Acinar Cells in CD Rats. rats nearly normalize. Federal government websites often end in .gov or .mil. Intact ventricular myocytes from MyBP-C(+/-) hearts displayed abnormal sarcomere shortening but unchanged Ca(2+) transient kinetics. We focus first on the thrombotic and thrombolytic serine proteases and known interactions between these protease cascades and elements of the innate immune response. Early murine cholestatic liver injury is via autophagy pathway. Sprague Dawley rats; Pharmacological interventions; Toxicological effects. Blood, 56 (5) (1980). Rats were, Objective: We find that the biogenesis of mitochondria is response to the early stage of cholestasis with internal pathway. Sprague-Dawley, Inc. Harlan became Envigo in 2015. This study aimed to validate an analytical method to determine DNA concentration using standard reference material (NIST SRM 2372) and Sprague Dawley rat and human DNA. Weanling rats The Hsd:Sprague Dawley ® SD rat has a wide array of historical research use including in the field of cardiology. In the skeletal muscle, the ageing process is characterized by a loss of muscle mass and strength, coupled with a decline of mitochondrial function and a decrease of satellite cells. Sunitinib-induced cardiotoxicity in 3, 12 and 24 months old male Sprague-Dawley rats and MKK7 expression and activation was investigated using the Langendorff perfused heart model followed by Western blot analysis. 0000025125 00000 n 37 Full PDFs related to this paper. This article is protected by copyright. Species: Rat. However, the mechanism by which heart function declines and heart failure increases in association with age is not clear. Lower panel: Representative immunoblot images of Dynamin Like Protein-1 (Dlp1), Mitochondrial fission 1 protein (Fis1), Mitofusin-2 (Mfn2), Dynamin-like 120kDa protein (OPA1), Cyclophilin D (CypD), and NAD-dependent protein deacetylase sirtuin-3 (Sirt3) in cardiac muscles of young (white bars), old adult (gray bars) and senescent (black bars) rats. Specifically, NMR metabonomics profiling of serum revealed that centenarians are characterized by a metabolic phenotype distinct from that of elderly subjects, in particular regarding amino acids and lipid species. 14 0 obj <> endobj xref 14 30 0000000016 00000 n November 2018 . – Chiu, T. Spontaneous Hypertrophic Foci of Pancreatic Acinar Cells in CD Rats. Proteins significantly altered (ANOVA and Tukey, p<0.01) are expressed as a ratio of spot volume variations in old adult and senescent rats versus young (6 months) control, respectively. Toxicologic Pathology, 11 (2) (1983). Anti-cancer drug Sunitinib is linked to adverse cardiovascular events, which have shown to involve mitogen activated kinase kinase 7 (MKK7) pathway. 0000004719 00000 n The typical life span is 2.5–3.5 years. 0000005054 00000 n This breed of rat was first produced by the Sprague Dawley farms in Madison, Wisconsin (later to become the Sprague Dawley Animal Company). I am a model organism used by Duke University to research the impacts of chemical exposure on behavior, memory, and cognitive ability. Blood, 56 (5) (1980). Considering the particular actions of these metabolites, these data are suggestive of a better counteractive antioxidant capacity and a well-developed membrane lipid remodelling process in the healthy aging phenotype. The mRNA expression levels of LC3B and SQSTM1 increased in the OA and OAE groups, with a significant difference observed between the two groups, while a concomitant decrease was detected in BECLIN1 levels. Among the five cysteine residues of actin, Cys(239) and Cys(259) were oxidized to sulfenic (Cys-SOH), sulfinic (Cys-SO(2)H), or sulfonic (Cys-SO(3)H) acids by increasing amounts over the time periods studied. We designed a study using a murine model involving the transplantation of autologous ASCs in rats subjected to burn injuries. Lower panel: Representative immunoblot images of BCL2/adenovirus E1B 19kDa protein-interacting protein 3 (Bnip3), Beclin-1, apoptosis regulator Bcl-2 (Bcl2), the phosphorylated/total amount ratio of Serine/threonine-protein kinase mTOR (P-mTor/mTor), cytosolic (18kDa) and lipidated (16kDa) forms of Microtubule-associated proteins 1A/1B light chain 3B (LC3), in cardiac muscles of young (white bars), old adult (gray bars) and senescent (black bars) rats. AT 900 MHz IN Hsd:SPRAGUE DAWLEY SD RATS (WHOLE-BODY EXPOSURE) Scheduled Peer Review Date: March 26 to 28, 2018 NOTICE This DRAFT Technical Report is distributed solely for the purpose of predissemination peer review under the applicable information quality guidelines. Sprague–Dawley rats, born in the laboratory.were maintained under conditions of controlled temperature and lighting (12 h-12 h) with free access to food and water. Biochemical analysis of mitochondrial plasticity. Sprague Dawley Inc. α-LA may ameliorate oxidative stress through up-regulating ALDH2 activity in patients with ACS. Dawley ® Rat. Taconic received stock from the NIH Animal Genetic Resource in 1970. Burn scar pain is considered as neuropathic pain. 1990). The pure computationally inferred model presented in the paper is therefore a good candidate to represent the relationship between key genes and miRs involved in cardiac senescence processes, and represents a reliable selection of genes and miRs for further studies, in order to elucidate and better detail their involvement in cardiac aging. Blood was collected by decapitation from 3000 rats between 12.00 and 13.00. Haemodynamic alterations were observed following Sunitinib administration. No bodyweight difference was observed between groups during the study. Laboratory Animals, 24, 253-264 (1990). National Toxicology Program . Its main advantage is its calmness and ease of handling. 0000003429 00000 n Two groups of Sprague Dawley and 2 groups of Lister-Hooded rats were controls for the gavage and diet administration. The Sprague Dawley rat and Long–Evans rat were developed from Wistar rats. Sprague-Dawley, Inc. Harlan became Envigo in 2015. Why are you such a great model? (V/m)a I 0 M 412 F 405 M+F 817 II 5 M 401 F 410 M+F 811 III 25 M 209 F 202 M+F 411 IV 50 M 207 F 202 M+F 409 Total 2448 a Treatment with GSM-RFR 1.8GHz for 19h/day started on the 12th day of pregnancy and lasted … A total of 30 male Sprague‑Dawley rats were randomly divided into three groups (n=10), including the control, OA and OA with treadmill exercise (OAE) groups. Then, sampling was done from rats liver. Sprague–Dawley rat pups of postnatal day (P) 10 or 20 were subjected to generalized and sustained convulsions (>20 min duration, a model for status epilepticus, SE), provoked by i.p. Results: Statistical analysis was performed by Student’s T-Test (n=3; * p<0.05). evaluated upon completing the treadmill exercise program (speed, 18 m/min; 30 min/day; 5 days/week for 4 weeks). TBP was well absorbed and metabolized to form glucuronide and sulfate conjugates after oral (0.1–1000 µmol/kg) and dermal (100 nmol/cm 2) administration with significant systemic bioavailability in Sprague Dawley (SD) rats and B6C3F1/Tac mice (Knudsen et al., 2019). 0000002458 00000 n 360 Sprague-Dawley rats (179 males and 181 females) and a 26% incidence was seen in 254 Swiss mice (101 males and 153 females) used as untreated control animals in an 18-month series of carcinogenesis experiments. H�\�͎�@��. demonstrated that elements of heart protection could be present in disease-free ageing of Sprague-Dawely rats. Coat Color Loci: Tyr c. Color: Albino. International Journal of Molecular Sciences. A total of 30 male Sprague‑Dawley rats were randomly divided into three groups (n=10), including the control, OA and OA with treadmill exercise (OAE) groups. On the contrary, inhibition of autophagy by treating with chloroquine exacerbated blood-spinal cord barrier permeability, promoted the loss of TJ proteins and enhanced ER stress after spinal cord injury. sativus ethanolic extract as a hypoglycemic agent on Sprague-Dawley rats randomly divided into five groups of five induced to diabetes using alloxan (120 mg/kg body weight intraperitoneal). 0000000896 00000 n In particular, gastrocnemius displayed a limited number of dysregulated proteins in adult mice, while in aged muscles the modifications were more pronounced in terms of number and level. The rats … The STZ-induced group received 35 mg / kg STZ by intraperitoneal injection while the STZ and HFD-induced group received the STZ after 14 days high fat diet treatment. The content of reactive carbonyl-groups increased 4-fold within the studied 24 h period. Wistar rats are more active than others like Sprague Dawley rats. clarify the relationships between ASCs, scar pain, apoptosis and autophagy. It has not been formally disseminated by the NTP. %PDF-1.4 %���� At day 1 after spinal cord injury, blood-spinal cord barrier was disrupted and activation of ER stress and autophagy were involved in the rat model of trauma. Try out the SAS Sprague Dawley rat with our animal model evaluation program→ In addition, a significant increase was observed between OA and OAE groups in LC3B and SQSTM1 protein levels, whereas no change was observed in BECLIN1 levels between the OA and OAE groups in the superficial and deep zones. However, an in vivo phototoxicity test method to evaluate this has not been … Before sharing sensitive information, make sure you're on a federal government site. *Significant differences (Student’s t-test, n=3, p < 0.05). 0000001646 00000 n Sepsis in Sprague-Dawley Rats. Azienda Ospedaliero Universitaria Maggiore della Carità, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International, Mitochondrial Function and Mitophagy in the Elderly: Effects of Exercise, Ageing alters the severity of Sunitinib-induced cardiotoxicity: Investigating the mitogen activated kinase kinase 7 pathway association, Collagen VI Null Mice as a Model for Early Onset Muscle Decline in Aging, In-silico cardiac aging regulatory model including microRNA post-transcriptional regulation, A computationally inferred regulatory heart aging model including post-transcriptional regulations, Mitochondria hyperfusion and elevated autophagic activity are key mechanisms for cellular bioenergetic preservation in centenarians, Serum profiling of healthy aging identifies phospho- and sphingolipid species as markers of human longevity. h�b``�```~����P����x�b�,3�[[2�����zl3 ��� Sli@�@'#���bY�\/� TBP is detected in environmental matrices and biota, including human breast milk, placenta, and serum. In summation, the group B animals were exposed to HFHC diet supplemented with 15% fructose for 20 weeks and designated as the prediabetic group (PD) while … This appears to be due to a decreased mitophagy, induced by hyperfused, elongated mitochondria. Collagen VI is an extracellular matrix (ECM) protein playing a key role in skeletal muscles and whose deficiency leads to connective tissue diseases in humans and in animal models. Despite a lack of left ventricular hypertrophy, MyBP-C(+/-) hearts exhibited elevated end-diastolic pressure and decreased dP/dtmax, which was normalized following dobutamine infusion. 0000005893 00000 n To address reports of TBP accumulation in human placenta and breast milk, studies were conducted to characterize TBP disposition and toxicokinetics in timed-pregnant or nursing Sprague Dawley (SD) rats following a single oral dose to the dam. 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